This study was conducted with funding from the Danish International Development Agency (Danida) provided specifically to prepare for the launch of Oxfam education projects in Pibor and Juba in South Sudan. However, its findings will also be useful to the wider NGO community working in the country, with recommendations provided for the Government of South Sudan and for future programming by donors. The analysis focuses specifically on education, but it also aims to analyse gendered power relations between men and women and boys and girls and the differences in their roles and responsibilities, decision-making power, the barriers and constraints they face and their coping mechanisms, along with the specific needs and concerns of girls and boys both in and out of school and gendered vulnerabilities and differential access to education in the locations selected. The analysis concludes with a set of recommendations to ensure that agencies can move forward in a way that meaningfully addresses the gender inequalities that prevent access to their programmes for women, men, boys and girls.